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为了探讨鸭SREBP1基因对前列腺癌PC3细胞增殖的影响,通过构建携带His标签鸭SREBP1基因第310位至403位氨基酸序列的真核表达载体p EGFP-N3+SREBP1+His,转染前列腺癌PC3细胞,空载体p EGFP-N3为对照,结果表明:p EGFP-N3+SREBP1+His和空载体均成功转染前列腺癌PC3细胞,p EGFP-N3+SREBP1+His转染效率高于空载体,通过检测转染48 h的细胞数量和SREBP1表达量,p EGFP-N3+SREBP1+His组细胞数为0.82×10~6个,SREBP1基因表达量为124.128 pg/m L;空载体组细胞数为0.74×10~6个,没有检测到His标签表达量。研究结果揭示,鸭SREBP1基因第310位至403位氨基酸序列对前列腺癌PC3细胞的增殖有促进作用。
To investigate the effect of SREBP1 gene on the proliferation of prostate cancer PC3 cells, prostate cancer PC3 cells were transfected with pEGFP-N3 + SREBP1 + His, an eukaryotic expression vector carrying the 310th to 403th amino acids of SREBP1 gene of His-tagged duck The results showed that p EGFP-N3 + SREBP1 + His and empty vector were successfully transfected into PC3 cells, and the transfection efficiency of p EGFP-N3 + SREBP1 + His was higher than that of empty vector. The number of transfected cells and the expression of SREBP1 were detected 48 h after transfection. The number of cells in p EGFP-N3 + SREBP1 + His group was 0.82 × 10 ~ 6, the expression of SREBP1 gene was 124.128 pg / m L, and the number of cells in empty vector group was 0.74 × 10 ~ 6, His Tag expression was not detected. The results revealed that amino acids 310 to 403 of duck SREBP1 gene promoted the proliferation of prostate cancer PC3 cells.