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目的通过生物信息学,预测菲律宾蛤仔原肌球蛋白的线性表位。方法对菲律宾蛤仔原肌球蛋白同源性、抗原性指数进行分析,利用在线工具预测及综合分析预测表位,进一步与人和猪原肌球蛋白序列对比初步筛选过敏原线性表位。结果 4种软体类的原肌球蛋白同源性较高,且原肌球蛋白的整体性抗原指数较高。菲律宾蛤仔原肌球蛋白中抗原表位区域较多,在与人、猪的序列比较后,最终预测出4个抗原表位。结论预测出了4个线性表位分别为Peptide 2 TLLQKKYTNLENEFDQVNEK,Peptide 6 EKQVKDAKYVAEEADRKYDE,Peptide 9 KDAENRAAEAERVVNKLQ和Peptide 10 ELLAEKEK YKAISDELDQ。
Objective To predict the linear epitope of tropomyosin of Ruditapes philippinarum by bioinformatics. Methods The tropomyosin homology and antigenicity index of Ruditapes philippinarum were analyzed. The online tools were used to predict and analyze the predicted epitopes. The linear epitopes of allergens were further screened by comparing with the human and porcine tropomyosin sequences. Results The tropomyosin homology of the four species was higher, and the tropism of the tropomyosin was higher. There are many antigenic epitopes in clam tropomyosus of Philippines and 4 antigenic epitopes are finally predicted after comparison with human and porcine sequences. Conclusion Four linear epitopes were predicted as Peptide 2 TLLQKKYTNLENEFDQVNEK, Peptide 6 EKQVKDAKYVAEEADRKYDE, Peptide 9 KDAENRAAEAERVVNKLQ and Peptide 10 ELLAEKEK YKAISDELDQ, respectively.