论文部分内容阅读
目的 :观察雄性家兔下丘脑弓状核核团的神经电活动及其在性成熟前后的变化。方法 :依据出生时间和睾丸大小确定雄性家兔的性发育成熟。选择性发育成熟前雄性家兔 8只和已发育成熟的雄性家兔 7只 ,采用核团微穿刺记录放电的电生理技术 ,比较两组动物的下丘脑弓状核核团放电。并经过脑组织切片证实微电极正确定位于弓状核。结果 :经切片证实微电极准确定位于弓状核的 1 0只 (占 6 7% )。去除未准确定位的放电记录后 ,性成熟前的雄兔下丘脑弓状核放电频率明显少于性成熟后的雄兔 ,但最大脉冲幅度差异不显著。结论 :下丘脑弓状核是调节合成与分泌促性腺激素释放激素 ( Gn RH)的最主要核团 ,其电生理活性表达、尤其是脉冲频率与雄性兔的性成熟相关
Objective: To observe the neural activity of hypothalamic arcuate nucleus in male rabbits and their changes before and after sexual maturation. Methods: According to the birth time and testicular size to determine the sexual maturity of male rabbits. Eight premature male rabbits and seven mature male rabbits were selectively developed. Electrophysiological techniques were used to record the discharge of the nucleus by micro-puncture. The arcuate nucleus of the hypothalamus of two groups were compared for discharge. And confirmed by brain tissue biopsy microelectrode located in the arcuate nucleus. Results: It was confirmed by biopsy that 10 electrodes (67%) were accurately located in arcuate nucleus. The discharge frequency of hypothalamic arcuate nucleus of pre-mature male rabbits was significantly less than that of sexually mature male rabbits after removing the imprecise discharge records, but the maximum pulse amplitude was not significantly different. Conclusions: The hypothalamic arcuate nucleus is the most important nucleus that regulates the synthesis and secretion of gonadotrophin-releasing hormone (Gn RH). Its electrophysiological activity, especially the pulse frequency, is related to sexual maturation in male rabbits