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目的观察阿托伐他汀对脂多糖(LPS)诱导的THP-1巨噬细胞炎症因子分泌的影响,并探讨其机制。方法 100 nmol/L佛波酯孵育THP-1细胞24 h,使其分化为巨噬细胞后,换无血清培养基,加入LPS和(或)阿托伐他汀进行处理。酶联免疫吸附法检测细胞上清液中白细胞介素1β(IL-1β)和白细胞介素18(IL-18)含量,荧光定量PCR检测细胞核苷酸结合寡聚化结构域样受体蛋白1(NLRP1)炎性体的mRNA表达,Western blot检测细胞NLRP1炎性体的蛋白表达。结果阿托伐他汀可呈浓度、时间依赖性抑制LPS诱导的THP-1巨噬细胞IL-1β和IL-18释放;阿托伐他汀可下调THP-1巨噬细胞NLRP1炎性体mRNA和蛋白的表达。结论阿托伐他汀抑制巨噬细胞炎症因子分泌,其作用机制可能与其下调NLRP1炎性体表达有关。
Objective To observe the effect of atorvastatin on the secretion of inflammatory cytokines induced by lipopolysaccharide (LPS) in THP-1 macrophages and its mechanism. Methods THP-1 cells were incubated with 100 nmol / L phorbol ester for 24 h. After differentiated into macrophages, the cells were treated with LPS and / or atorvastatin in serum-free media. The contents of IL-1β and IL-18 in the supernatant of the cells were detected by enzyme-linked immunosorbent assay (ELISA), and the expression of IL-18 and IL-18 (NLRP1) inflammasome mRNA expression was detected by Western blot NLRP1 inflammasome protein expression. Results Atorvastatin could inhibit the LPS-induced release of IL-1β and IL-18 from THP-1 macrophages in a concentration-dependent and time-dependent manner. Atorvastatin down-regulated the mRNA and protein expression of NLRP1 in THP-1 macrophages expression. Conclusions Atorvastatin inhibits the secretion of inflammatory cytokines in macrophages, and its mechanism may be related to the down-regulation of NLRP1 inflammasome expression.