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目的：评价干扰素α（ＩＦＮα）和白细胞介素２（ＩＬ－２）联合激活的白血病缓解期骨髓对白血病细胞（Ｋ５６２细胞）的杀伤作用。方法：半固体集落培养法。结果：ＩＬ－２激活３天的白血病缓解期骨髓对Ｋ５６２细胞有杀伤作用，杀伤对数级达０．１３～２．３０。ＩＦＮα可明显增强ＩＬ－２激活骨髓的杀伤作用，其对Ｋ５６２细胞的杀伤率可进一步提高，达０．３０～＞３．１５对数级，ＩＬ－２和ＩＦＮα联合对骨髓ＣＦＵ－ＧＭ和Ｋ５６２细胞无明显抑制作用。小剂量ＩＬ－２与ＩＦＮα两者联合比它们单独作用于已激活骨髓杀伤Ｋ５６２细胞的能力更强。结论：ＩＦＮα能增强ＩＬ－２激活的白血病缓解期骨髓对Ｋ５６２细胞的杀伤作用；持续加小剂量ＩＬ－２和ＩＦＮα能更有效地维持激活骨髓对Ｋ５６２细胞的杀伤效能
OBJECTIVE: To evaluate the killing effect of bone marrow on leukemia cells (K562 cells) during remission by leukemia activated by interferon alpha (IFNα) and interleukin 2 (IL-2). Methods: Semi-solid colony culture method. Results: The myeloid leukemia cells that had been activated by IL-2 for 3 days had a lethal effect on K562 cells, with a logarithmic scale of 0.13-2.30. IFNα significantly enhanced the killing effect of IL-2-activated bone marrow, and its killing rate on K562 cells could be further increased to 0.30 ~> 3.15 logarithm level. The effect of IL-2 and IFNα on bone marrow CFU-GM and K562 No significant inhibition of cells. The combination of low-dose IL-2 with IFN [alpha] is more potent than their ability to act alone on activated K562 cells. CONCLUSION: IFNα can enhance the killing effect of bone marrow on K562 cells during remission of IL-2-activated leukemia. It is more effective to maintain the killing efficacy of activated bone marrow on K562 cells with continuous addition of small doses of IL-2 and IFNα