应用不依赖于培养方法的分子技术,研究堆肥中真菌群落的组成及其随温度变化的现象,正在修正以往人们对堆肥过程中重要真菌类群的认识。另外,新一代测序技术的利用虽有限,但已经较先前开始时揭示出更大、更多样化的菌物群落特异性。嗜热菌物种的数量,潜在地促进了先前研究的非特异性嗜热菌物种被大量发现,这些菌物可能具有重要的生物技术应用潜能。 The application of molecular techniques that do not depend on culture methods to study the composition of fungal communities in composting and their effect on temperature is ameliorating the previous understandings of important fungal groups in composting. In addition, the use of next-generation sequencing techniques is limited, but larger and more diverse bacterial community-specificities have been revealed earlier than previously. The number of thermophilic species, which has potentially contributed to the extensive discovery of non-specific thermophilic species previously studied, may have important potential for biotechnological applications.