目的建立同时测定化妆品中6种抗角化异常药物含量的双波长高效液相色谱法。方法样品经甲醇超声提取,采用Waters symmetry C_(18)柱(250 mm×4.6mm,5 mm)为色谱柱;甲醇-0.025 mol/L磷酸二氢钠缓冲液(p H 5.20)为流动相,梯度洗脱;流速为1.2 mL/min;柱温35.0℃;二极管阵列检测器检测,检测波长为355nm和269 nm。结果各待测物质分离适中且线性良好,相关系数r≥0.9999;在空白样品中添加3个水平的标准品,回收率均在91.2%~103%,相对标准偏差均小于3.8%(n=3);6种标准溶液室温放置48 h均稳定;方法的检出限为0.3~2.5 mg/kg,定量限为0.9~7.5 mg/kg。结论该方法准确、快捷、灵敏度高、分离度好、选择性好,适用于化妆品中6种非法添加的抗角化异常药物的同时测定。 Objective To establish a dual-wavelength high performance liquid chromatography (HPLC) method for simultaneous determination of six anti-keratinization drugs in cosmetics. Methods The sample was extracted by methanol and eluted with a Waters symmetry C_ (18) column (250 mm × 4.6 mm, 5 mm). The mobile phase was methanol-0.025 mol / L sodium dihydrogen phosphate buffer (pH 5.20) Gradient elution; flow rate of 1.2 mL / min; column temperature 35.0 ℃; diode array detector detection, detection wavelength of 355nm and 269nm. Results The analytes were well-separated and linear with a correlation coefficient of r ≥0.9999. The recoveries of three standard samples were 91.2% -103% with relative standard deviations (RSDs) less than 3.8% (n = 3 ). The six standard solutions were stable at room temperature for 48 h. The detection limits were 0.3-2.5 mg / kg and the limits of quantification were 0.9-7.5 mg / kg. Conclusion The method is accurate, rapid, sensitive, good separation and good selectivity. It is suitable for the simultaneous determination of 6 kinds of anti-keratosis abnormal drugs illegally added in cosmetics.