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目的用高效液相色谱法测定2型糖尿病患者血清中五种磷脂含量的变化。方法取70例2型糖尿病患者血清、81例正常对照组人群血清;采用正向高效液相色谱法测定血清中的磷脂酰丝氨酸(PS)、磷脂酰肌醇(PI)、磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、神经鞘磷脂(SM)的含量,并做对比研究。结果 2型糖尿病患者体内PS[(0.139±0.031)mg/L vs.(0.154±0.029)mg/L]、PI[(0.021±0.016)mg/L vs.(0.027±0.017)mg/L]、PC[(0.150±0.039)mg/L vs.(0.385±0.022)mg/L]、PE[(0.070±0.026)mg/L vs.(0.087±0.044)mg/L]含量均低于正常人,差异有统计学意义(均P<0.05),SM含量[(0.039±0.026)mg/L vs.(0.044±0.028)mg/L]与正常人比较差异无统计学意义(P>0.05)。结论 2型糖尿病患者血清中PS、PI、PC、PE含量呈现下降趋势,提示这四种指标对糖尿病患者在正常人群中的筛查起到了辅助性作用。
Objective To determine the changes of five phospholipids in serum of type 2 diabetic patients by high performance liquid chromatography. Methods Seventy patients with type 2 diabetes mellitus and 81 normal controls were enrolled in this study. Serum levels of phosphatidylserine (PS), phosphatidylinositol (PI) and phosphatidylcholine PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) were measured and compared. Results PS [(0.139 ± 0.031) mg / L vs. (0.154 ± 0.029) mg / L], PI [(0.021 ± 0.016) mg / L vs. (0.027 ± 0.017) mg / L] in patients with type 2 diabetes, PC (0.150 ± 0.039) mg / L vs. (0.385 ± 0.022) mg / L] and PE [(0.070 ± 0.026) mg / L vs. (0.087 ± 0.044) mg / L] The difference was statistically significant (both P <0.05). There was no significant difference in SM content [(0.039 ± 0.026) mg / L vs. (0.044 ± 0.028) mg / L vs normal controls) (P> 0.05). Conclusions The serum levels of PS, PI, PC and PE in type 2 diabetic patients showed a decreasing trend, suggesting that these four indexes play an auxiliary role in the screening of diabetic patients in normal population.