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目的 探讨不同组织类型胃癌局部免疫细胞及细胞因子表达的特性。方法 采用高敏感放射性标记半定量逆转录 聚合酶链反应 (RT PCR)技术检测胃癌患者癌组织及非癌性黏膜组织中不同T细胞亚群 2 4个亚家族的T细胞受体 β链可变区表达以及T细胞亚群、上皮细胞的细胞因子表达谱。结果 弥散型胃癌组织CD8+ T细胞亚群中增殖性T细胞克隆数目明显低于肠型胃癌 (P =0 0 46 )。弥散型胃癌中 ,癌组织CD8+ T细胞中白细胞介素 (IL) 6、IL 8、CD4+ T细胞中肿瘤坏死因子(TNF) αmRNA的水平 (0 6 1± 0 2 9,0 5 6± 0 2 2 ,0 0 9± 0 0 3)较非癌性黏膜组织中的 (0 14± 0 0 5 ,0 2 7± 0 0 9,0 0 4± 0 0 2 )增高 (P =0 0 2 8,P =0 0 43,P =0 0 46 ) ;而肠型胃癌中 ,癌组织CD8+ T细胞及上皮细胞中IL 8mRNA的水平 (0 5 7± 0 2 5 ,0 2 7± 0 0 7)较非癌性黏膜组织中的 (0 2 1± 0 0 7,0 14± 0 0 6 )增高 (P =0 0 2 8,P =0 0 2 8)。结论 不同组织类型胃癌局部免疫细胞及细胞因子表达的特性不同 ,弥散型胃癌中增殖性T细胞克隆少、局部多种免疫抑制型细胞因子的高表达提示 :弥散型胃癌肿瘤局部的免疫抑制比肠型胃癌更严重。
Objective To investigate the characteristics of local immune cells and cytokines expression in different types of gastric cancer. Methods High sensitive radiolabeled semiquantitative RT-PCR (RT-PCR) was used to detect T cell receptor beta chain variable in 24 subfamilies of different T cell subsets in gastric cancer tissues and non-cancerous mucosa tissues Region expression and T cell subsets, epithelial cells cytokine expression profiles. Results The number of proliferating T cell clones in CD8 + T cell subsets of diffuse gastric cancer was significantly lower than that of intestinal type gastric cancer (P = 0.046). The levels of tumor necrosis factor (TNF) αmRNA in interleukin (IL) 6, IL 8 and CD4 + T cells in CD8 + T cells of diffuse type gastric cancer (0 6 1 ± 0 2 9, 0 5 6 ± 0 2 2,09 ± 0 0 3) were significantly higher than those in non-cancerous mucosa (0 14 ± 0 0 5, 0 2 7 ± 0 0 9,0 0 4 ± 0 0 2) (P 0 028 , P = 0 0 43, P = 0 0 46). However, the levels of IL 8 mRNA in CD8 + T cells and epithelial cells in intestinal cancer (0 57 ± 0 25, 0 27 ± 0 0 7) (0 2 1 ± 0 0 7,0 14 ± 0 0 6) in non-cancerous mucosa (P = 0 0 28, P = 0 0 28). Conclusions The characteristics of local immune cells and cytokines expression in different types of gastric cancer are different. There are few proliferating T cell clones in diffuse gastric cancer, and the high expression of local immunosuppressive cytokines suggests that local immunosuppression of diffuse gastric cancer is more severe than that of intestinal Gastric cancer is more serious.