本研究首次报道了赫坎按蚊种团内亲缘种间基因组中重复DNA限制酶切长度差异。研究的蚊种包括中华按蚊、嗜人按蚊、凉山按蚊、克劳按蚊和小宽按蚊等5个亲缘种。制备每种蚊的基因组DNA,分别以BglⅡ、HaeⅡ和PstⅠ等3种限制性核酸内切酶消化,琼脂糖电泳分离,比较重复序列DNA的带型.结果显示5个亲缘种的重复DNA的限制酶切片段的带型有明显的差异.该法敏感可靠,可作为亲缘种间鉴别的一个方法。 This study, for the first time, reported the differences in the length of repeat DNA restriction endonucleases among the relatives of the ancestrous species within the Hercian group. The mosquito species studied include Anopheles sinensis, Anopheles anthropophagus, An. Liangshan Anopheles, Anopheles claus and An. The genomic DNA of each mosquito was prepared and digested with restriction endonucleases BglII, HaeII and PstI and separated by agarose gel electrophoresis to compare the bands of repeat DNA.The results showed that the restriction of repetitive DNA in 5 relatives The bands of the digested fragments have obvious differences.The method is sensitive and reliable and can be used as a method of identification between relatives.