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  5. Effects of Glycerol in the Refolding and Unfolding of Creatine Kinase

Effects of Glycerol in the Refolding and Unfolding of Creatine Kinase

来源 :清华大学学报 | 被引量 : 0次 | 上传用户：yyuan
【摘 要】
：
The effects of glycerol in the refolding, reactivation, unfolding, and inactivation of guanidine- denatured creatine kinase were studied by observing the fluore
【作 者】
：
欧文斌 朴龙斗 孟凡国 周海梦 
【机 构】
：
Department of Biological Sciences and Biotechnology
【出 处】
：
清华大学学报
【发表日期】
：
2004年期
【关键词】
：
glycerol creatine kinase unfolding refolding guanidine denaturation 
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The effects of glycerol in the refolding, reactivation, unfolding, and inactivation of guanidine- denatured creatine kinase were studied by observing the fluorescence emission spectra and the circular dichroism spectra, and by recovery and inactivation of enzymatic activity and aggregation. The results show that low concentrations of glycerol (
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