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含有单体牛细胞色素c氧化酶的脂质双层膜,被成功地固定在金石英晶体微平衡电极上.在较宽的pH值、温度和缓冲浓度范围内观察到了氧化酶修饰电极上的直接电子迁移.氧化酶修饰电极在80°C以上时保持直接电子迁移性质,将电极冷却到室温时,氧化酶仍保持电子迁移能力.在22～80°C范围内,温度变化可引起细胞色素c氧化酶的相转移,估计出了相转移前后的相应反应活化能通过射流分析,研究了在溶液含有亚铁细胞色素c氧化酶的电氧化过程中,乙腈同细胞色素c氧化酶的结合.结果显示:浓度1.3 M具有常数Ki时,细胞色素c氧化酶和乙腈形成配合物,每个细胞色素c氧化酶配一个乙腈分子,乙腈同细胞色素氧化酶的结合过程是可逆过程.
Lipid bilayer membranes containing monomeric bovine cytochrome c oxidase were successfully immobilized on gold-quartz crystal microbalance electrodes.On the wide range of pH, temperature and buffer concentrations, Direct electron transport. Oxidation of the modified electrode at 80 ° C above the direct electron mobility to maintain the nature of the electrode is cooled to room temperature, the oxidase remains electron transfer ability in the range of 22 ~ 80 ° C, the temperature can cause cytochrome c oxidase phase transfer, estimated the corresponding reaction activation phase before and after the phase transition by jet analysis of the solution contains ferrous cytochrome c oxidase oxidation process, the combination of acetonitrile and cytochrome c oxidase. The results showed that when the concentration of Ki was constant at 1.3 M, the cytochrome c oxidase formed a complex with acetonitrile, and one acetonitrile molecule per cytochrome c oxidase. The binding of acetonitrile to cytochrome oxidase was reversible.