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采用聚丙烯酰胺凝胶圆盘电泳方法，研究了正常草鱼和患出血病草鱼的肾脏、肝脏、脾脏、心脏、鳃、肌肉以及血清中的苹果酸脱氢酶（ＭＤＨ）同工酶。结果表明，患病草鱼和正常草鱼各组织器官及血清中的ＭＤＨ同工酶酶谱均为６条带，患病鱼的酶谱明显地不同于正常鱼的酶谱；ＭＤＨ同工酶的活性具有组织和器官的特异性，同一组织及器官，患病草鱼ＭＤＨ同工酶的总活性较正常草鱼偏高，其中鳃、肝脏、脾脏以及血清中的ＭＤＨ同工酶总活性具有显著性差异（ｐ＜０．０５）；同种组织及器官，患病草鱼的ＭＤＨ同工酶的相应组分，活性序列产生了较大的差异；ＭＤＨ同工酶可以作为草鱼患出血病的一个生理、病理指标。
The MDH isozymes in kidney, liver, spleen, heart, gill, muscle and serum of grass carp and hemorrhagic grass carp were studied by polyacrylamide gel electrophoresis. The results showed that there were 6 bands of MDH isozymes in tissues and organs of sick grass carp and normal grass carp. The zymogram of diseased fish was significantly different from that of normal fish; the activity of MDH isozymes The total activities of MDH isozymes in the same tissues and organs, grass carp and diseased grass carp were higher than that in normal grass carp. The total MDH isozymes in gill, liver, spleen and serum were significantly different p <0.05). The corresponding components and active sequences of MDH isozymes in the same tissues, organs and diseased grass carp had a great difference. MDH isozymes could be used as a physiological and pathological index.