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目的:探讨外源性双酚A(BPA)调控MCF-7乳腺癌细胞迁移的分子机制。方法:通过划痕实验和Transwell实验检测MCF-7乳腺癌细胞迁移能力,并以黏附实验反向辅助验证;通过质粒转染干扰Snail蛋白表达水平,进一步研究外源性BPA调控MCF-7乳腺癌细胞迁移的分子机制。结果:外源性BPA能上调细胞中Snail的表达水平,从而下调E-cadherin的表达并促进MCF-7乳腺癌细胞迁移;siRNA干扰Snail表达后MCF-7乳腺癌细胞迁移减慢。此外,BPA刺激能降低MCF-7乳腺癌细胞黏附能力。结论:外源性BPA可通过上调Snail表达水平,促进MCF-7乳腺癌细胞的迁移,为进一步阐明乳腺癌细胞侵袭与转移的分子调控机制提供了新线索。
Objective: To investigate the molecular mechanism of exogenous BPA in the regulation of MCF-7 breast cancer cell migration. Methods: The migration ability of MCF-7 breast cancer cells was detected by scratch test and Transwell assay. The adhesion assay was used to reversely verify the expression of Snail protein. The expression of Snail protein was also interfered by plasmid transfection, and the effect of exogenous BPA on MCF-7 breast cancer The molecular mechanism of cell migration. Results: Exogenous BPA up-regulated the expression of Snail, down-regulated the expression of E-cadherin and promoted the migration of MCF-7 breast cancer cells. The siRNA transfection inhibited the migration of MCF-7 breast cancer cells after Snail expression. In addition, BPA stimulation can reduce the adhesion of MCF-7 breast cancer cells. Conclusion: Exogenous BPA can promote the migration of MCF-7 breast cancer cells by up-regulating the expression of Snail and provide new clues for further elucidating the molecular regulation mechanism of breast cancer cell invasion and metastasis.