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目的观察移植人脐血间充质干细胞诱导分化的神经元样细胞是否可以促进大鼠脊髓损伤的功能恢复。方法 30只SD大鼠随机分成移植组和对照组。建立NYU脊髓损伤模型。移植组移植Hoechst标记的源于脐血间充质干细胞在体外培养、扩增,向神经元样细胞分化的细胞,并用免疫荧光标记方法检测神经元特异性标记物。对照组注射PBS液。采用BBB评分标准对两组所有大鼠术前和术后24 h,1、2、3、4、5周进行运动功能评价,组织学和免疫组化分析移植细胞的定位和分化情况。结果经诱导后的脐血间充质干细胞表达神经丝蛋白和神经元特异性烯醇化酶,移植后在脊髓病理切片中出现Hoechst标记的移植细胞,5周后移植组功能恢复比对照组明显(P<0.05)。结论移植人脐血间充质干细胞诱导分化为神经元样细胞可以促进鼠脊髓损伤的功能恢复。
Objective To observe whether the transplantation of human umbilical cord blood-derived mesenchymal stem cells differentiate into neuron-like cells can promote the functional recovery of spinal cord injury in rats. Methods Thirty SD rats were randomly divided into transplantation group and control group. Establishment of NYU spinal cord injury model. Transplantation group transplantation Hoechst marker derived from umbilical cord blood mesenchymal stem cells cultured in vitro, expanded to differentiate into neuron-like cells, and immunofluorescence labeling method to detect neuron-specific markers. The control group was injected with PBS solution. BBB score was used to evaluate the motor function of all the rats in both groups before and 24 h, 1, 2, 3, 4 and 5 weeks after operation. Histology and immunohistochemistry were used to analyze the localization and differentiation of transplanted cells. Results The induced cord blood MSCs expressed neurofilament and neuron specific enolase. Hoechst labeled cells appeared in the pathological sections of the spinal cord after transplantation. After 5 weeks, the functional recovery of the transplantation group was more obvious than that of the control group P <0.05). Conclusion Transplantation of human umbilical cord blood derived mesenchymal stem cells into neuron-like cells can promote the functional recovery of spinal cord injury in rats.