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目的:应用核磁共振 (1H-NMR) 代谢组学方法研究有氧运动干预大鼠胰岛素抵抗 (insulin resistance, IR) 的作用机制.方法:2月龄大鼠40只, 随机分为正常饮食组和高脂饮食组喂养5周建模, 采用高胰岛素-正葡萄糖改良钳夹术对大鼠IR进行评价, 随之将其分为对照组 (N) 、模型组 (IR) 和模型运动组 (IRe) .IRe组有氧运动干预6周, 之后所有大鼠采用钳夹术评估IR, ELISA检测血清FINS, 1H-NMR测定大鼠胰腺组织代谢物, 多元统计分析代谢物谱差异, Metabo Analyst分析代谢通路, 受试者工作曲线 (ROC) 筛选潜在生物标志物.结果:1) 与N组相比, IR组FINS显著提高, GIR显著下降;与IR组相比, IRe组大鼠体重、FINS均显著下降, GIR显著性提高.2) 与N组相比, IR组胰腺组织中11个代谢物存在差异, 分别与6条代谢通路密切相关, ROC分析发现5个IR大鼠胰腺生物标志物.3) IRe组相对于IR组, 柠檬酸、谷氨酸、谷氨酰胺等5个生物标志物不同程度回调.结论:6周有氧运动可以显著改善高脂饮食诱导的大鼠IR状态, 其机制可能与有氧运动能够不同程度回调IR大鼠胰腺生物标志物、改善其涉及的代谢通路有关.“,”Objective: This study aimed at exploring the mechanism of aerobic exercise intervention on insulin resistance (IR) by1 H-NMR metabolomics. Methods: Forty 2-month-old rats were randomly divided into normal diet group and high-fat diet group for 5 weeks. IR Rats were evaluated by hyperinsulinemic-glucose improved clamp and then divided into control group (N), model group (IR) and model exercise group (IRe). The rats of IRe group finished 6 weeks aerobics. After the experiment, all IR rats were examined by clamp, serum FINS was tested by FINS, pancreatic tissue metabolites were explored by1 H-NMR and analyzed by multivariate statistical analysis, metabolic pathways were detected by Metabo Analyst, and potential biomarkers were screened by the ROC.Results: 1) Compared with N group, FINS in IR group increased significantly and GIR decreased significantly.Compared with IR group, the body weight and FINS of IRe group decreased significantly and GIR increased significantly. 2) Compared with N group, 11 metabolites in the pancreas tissue of the IR group were different and were closely related to the six metabolic pathways, and 5 IR pancreatic biomarkers were found by ROC analysis. 3) Compared with IR group, 5 biomarkers, such as citric acid, glutamic acid and glutamine, were changed to different degrees in IRe group. Conclusions: This 6-week aerobic exercise can significantly improve the IR induced by high-fat diet, and its mechanism may be related the fact that aerobic exercise can alter the pancreatic biomarkers in different degrees in IR rats and then improve their metabolic pathways.