目的探讨胰岛素样生长因子 Ⅰ (IGF Ⅰ )基因工程菌的摇瓶及发酵罐培养的一些发酵参数、诱导阶段的补料基质及诱导剂浓度对IGF Ⅰ表达的影响。方法通过摇瓶培养和分批及补料分批发酵培养进行研究。结果在摇床转速为 15 0、2 0 0、2 5 0r min条件下测得以葡萄糖为基准的Y△x △s分别为 0 .2 0 3、0 .2 7、0 .33。干细胞产量分别为 0 .5 4、0 .76 5、1.0 5 4g L。平均生长速率分别为 0 .0 34、0 .0 4 8、0 .0 6 6g (L·h)。细胞对数生长期分别为 6、7.5、10h。发酵罐上生物量由分批发酵的 18(A6 0 0nm)提高到补料分批发酵的 35 (A6 0 0nm) ,表达量占细胞可溶性总蛋白质的10 %。结论后期供纯氧与补料能延长对数生长期 ,提高乳糖浓度和诱导前用甘油作碳源有利于IGF Ⅰ表达 Objective To investigate some fermentation parameters of shake flask and fermentor culture of Insulin - like growth factor Ⅰ (IGF Ⅰ) genetically engineered bacteria, the effects of feed matrix and inducer concentration on the expression of IGF Ⅰ. Methods The shake flask culture and fed-batch fermentation were studied. Results The glucose-based Y △ x △ s measured at a speed of 15 0,2 0 0,2 5 0r min were 0.220, 0.27, 0.33 respectively. Stem cell yields were 0.54, 0.76 5 and 1.05 4g L, respectively. The average growth rates were 0 .0 34,0. 0 4 8,0. 0 6 6g (L · h). Cell logarithmic growth phase were 6,7.5,10 h. The biomass on the fermentor was increased from 18 (A6000nm) in batch fermentation to 35 (A6000nm) in fed-batch fermentation and the expression amount accounted for 10% of the total cell-soluble protein. CONCLUSIONS: Pure oxygen and feed supplement can prolong the logarithmic growth phase, increase lactose concentration and glycerol as carbon source before induction, which is beneficial to IGF Ⅰ expression