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在栽培平菇的培养基中添加适量的中药北芪粉所获得的子实体,经多年的食疗跟踪研究,对银屑病(牛皮癣)有一定作用,因其具有不同于普通平菇的特殊功效,所以特称“北芪菇”。为了对北芪菇开展更深入的研究,我们进行了深层培养添加北芪粉的研究。试图通过该试验为北芪菇的工业化生产奠定基础,进而为北芪菇从食疗向保健品或药品方面转化创造条件。 1 材料和方法 1.1 供试菌株 于菇忻干89,是从采自忻州的一株野生平菇分离所得。 1.2 培养基质 母种为PDA培养基。深层发酵为改良CM培养液:酵母膏0.5％,葡萄糖2％,玉米粉2％,豆饼粉1％,磷酸二氢钾0.1％,硫酸镁0.2％。 1.3 北芪粉加工 把成品中药北芪切成1cm大小,在钢磨上碾压成粉末,过细筛去渣,留粉末备用。 1.4 北芪粉添加量试验 每1000ml改良CM培养液中分别添加北芪粉5、10、15、20、25、30、35、40g共8组,每组重复3次。 1.5 菌丝体量 用80目铜丝网过滤发酵液,菌丝体经脱水烘干、称重,以评价发酵菌丝体产量。 1.6 多糖测定 用酚-硫酸法测多糖含量,并与普通
In the cultivation of Pleurotus ostreatus medium adding the appropriate amount of Chinese medicine Astragalus obtained fruiting body, after years of therapeutic follow-up study of psoriasis (psoriasis) has a certain role, because of its different from ordinary mushrooms special effects , So special called “Beiqi mushrooms.” In order to conduct a more in-depth study of Beiqi mushrooms, we conducted a deep culture study of adding Beiqi powder. Trying to lay the foundation for the industrialized production of Bertrich’s Mushroom by the test, thereby creating the conditions for Bertrandium mushrooms to be transformed from diet therapy to health products or medicine. 1 Materials and methods 1.1 The test strains in mushroom Xin stem 89, was collected from a wild mushroom Xinzhou isolated. 1.2 Cultivation of mother species of PDA medium. Submerged fermentation was modified CM medium: yeast extract 0.5%, glucose 2%, corn flour 2%, bean flour 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.2%. 1.3 Beiqi powder processing finished Chinese medicine Beiqi cut into 1cm size, rolled into a powder on the steel mill, fine sieve to slag, leaving the powder spare. 1.4 Beiqi powder addition test Each 1000ml modified CM culture were added Beiqi powder 5,10,15,20,25,30,35,40 g total of 8 groups, each group was repeated 3 times. 1.5 mycelium volume 80 mesh copper mesh filter fermentation broth, mycelium dehydration drying, weighing, in order to evaluate the yield of mycelium fermentation. 1.6 Determination of polysaccharide phenol - sulfuric acid method to determine polysaccharide content, and with ordinary