目的:建立毛郁金药材的HPLC指纹图谱方法,为其质量控制提供依据。方法:采用Agela Promosil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.5%冰乙酸水为流动相进行梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长250 nm,分析时间65 min。对10批毛郁金药材进行指纹图谱检测,并对不同产地的药材进行相似度评价和系统聚类分析。结果:建立了毛郁金药材的HPLC指纹图谱,确立了12个共有峰,相似度评价结果表明,各产地毛郁金药材相似度均>0.9。聚类系统分析将来自不同产地的10批药材分为3类。结论:该方法简便,快速,准确,可作为毛郁金药材的质量控制参考。 Objective: To establish HPLC fingerprinting method of MaoYuJin medicinal materials and to provide basis for its quality control. Methods: The gradient elution was carried out on an Agela Promosil C18 column (4.6 mm × 250 mm, 5 μm) using acetonitrile-0.5% glacial acetic acid as the mobile phase at a flow rate of 1.0 mL · min -1. The detection wavelength 250 nm, analysis time 65 min. Fingerprinting was performed on 10 batches of medicinal materials of tulipium, and similarity evaluation and cluster analysis of medicinal materials from different regions were conducted. Results: The HPLC fingerprints of Mao tulips were established and 12 common peaks were established. The results of similarity evaluation showed that the similarity of hair tulip medicinal herbs in each origin was> 0.9. Cluster Analysis The 10 batches of medicinal herbs from different origins were divided into three categories. Conclusion: The method is simple, rapid and accurate and can be used as quality control reference for hair tulip.