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通过利用RAPD(Random Amplified Polymorphic DNAs)随机引物中筛选到的可扩增出清晰条带、主带明显、稳定的10条引物,对来自安徽合肥、淮南、和县、潜山、岳西、江苏南京和四川邛崃等县市的发病辣椒上分离鉴定获得23个辣椒疫霉菌(Phytophthora capsici)进行全基因组DNA RAPD标记遗传多样性分析。选用引物共标出DNA指纹图带113条,其中多态性条带96条,多态性检测率为84.96%,表明辣椒疫霉菌具有较丰富的遗传多样性。根据引物扩增的DNA指纹图谱,运用UPGMA法分析,以遗传相似系数0.7为阈值,供试菌株被划分为3个遗传聚类组,除部分相同地理来源的菌株被划分为同一组外,其他不同菌株间的遗传相似性与地理来源无直接相关性。
By using RAPD (Random Amplified Polymorphic DNAs) random primers were screened to amplify a clear band, the main band with a clear, stable 10 primers, from Anhui Hefei, Huainan, and County, buried Hill, Yuexi, Jiangsu Nanjing, Sichuan Province, and other cities and counties to identify and identify Pepper. Phytophthora capsici 23 Phytophthora capsici genome-wide DNA RAPD marker genetic diversity analysis. A total of 113 DNA fingerprinting bands were selected, of which 96 bands were polymorphic. The detection rate of polymorphism was 84.96%, indicating that Phytophthora capsici has rich genetic diversity. According to the DNA fingerprinting amplified by primers, the genetic similarity coefficient of 0.7 was used as the threshold value by UPGMA analysis. The tested strains were divided into three genetic cluster groups, except that some of the same geographical origin strains were divided into the same group There is no direct correlation between the genetic similarity of different strains and geographical origin.