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目的观察高脂喂养及吡咯列酮干预对SD大鼠视黄醇结合蛋白4(RBP4)水平的影响。方法雄性SD大鼠随机分为3组,分别给予普通饮食(NC组)、高脂(F组)及高脂吡咯列酮(F+Pio组)干预,第8、12周时行OGTT及胰岛素释放试验(ITT),评价胰岛功能。检测FPG、血脂、肝功能及血清RBP4水平,并对肝脏、附睾脂肪和肾周脂肪称重,计算肝脏质量/体质量及脂肪质量/体质量。结果8周后,F组FPG、血脂、胰岛素及OGTT血糖曲线下面积(AUCOGTT)、AUCITT均高于NC组(P<0.05或P<0.01),血清RBP4水平升高(P<0.01);12周后,与F组相比,F+Pio组FPG、胰岛素、TG、TC、AUCOGTT、AUCITT、肝脏质量/体质量及脂肪质量/体质量和血清RBP4水平降低(P<0.05或P<0.01)。结论 PPAR-γ激动剂吡咯列酮能减轻糖脂毒性、改善IR并降低体内RBP4水平。
Objective To observe the effects of high fat diet and pioglitazone on the level of retinol binding protein 4 (RBP4) in SD rats. Methods Male Sprague-Dawley rats were randomly divided into 3 groups: normal diet (NC group), high fat (F group) and high fat pyrrolostone (F + Pio group) intervention. OGTT and insulin release test (ITT) to evaluate islet function. The levels of FPG, lipids, liver function and serum RBP4 were detected. The liver, epididymal fat and perirenal fat were weighed and the liver mass / body mass and fat mass / body mass were calculated. Results After 8 weeks, the areas under FPG, serum lipids, insulin and OGTT (AUCOGTT) and AUCITT in group F were significantly higher than those in group NC (P <0.05 or P <0.01) The levels of FPG, insulin, TG, TC, AUCOGTT, AUCITT, liver mass / body mass, fat mass / body weight and serum RBP4 level in F + Pio group were lower than those in F group (P <0.05 or P <0.01) . Conclusions The PPAR-γ agonist, pioglitazone, can reduce the glycolipid toxicity, improve the IR and reduce the level of RBP4 in vivo.