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硅是水稻的有益元素之一.针对位于水稻第6染色体短臂的谷壳硅含量QTLqHUS6.1,从前期建立的剩余杂合体衍生群体自交后代中,经目标区间的13个SSR标记检测,挑选出杂合区间彼此交迭的3个剩余杂合体,构建了3套近等基因系.在田间种植条件下,测量成熟后水稻谷壳、剑叶和茎秆的硅含量.表型分布和方差分析结果都显示,异质区间为RM19410~RM5815的近等基因系具有显著的基因型效应,而异质区间为RM4923~RM19410和RM19417~RM204的近等基因系未呈显著变异.经比较,将qHUS6.1定位在RM19410和RM19417之间约64.2kb,含9个候选基因的区域内,该QTL作用较强且同时控制水稻谷壳、剑叶和茎秆硅含量,增效等位基因来自父本密阳46,总体上呈加性遗传.本研究为qHUS6.1的克隆奠定了基础,并为QTL精细定位材料的构建和应用提供了新思路.
Silicon is one of the beneficial elements of rice.According to QTLqHUS6.1, which is located on the short arm of chromosome 6 of rice, the self-crossing progeny of the remaining hybrid population was detected by 13 SSR markers in the target interval, Three remaining hybrids were selected and their three near-isogenic lines were cloned, and the silicon content of mature rice husk, flag leaf and stalk were measured under field conditions.The phenotypic distribution and Analysis of variance showed that the near-isogenic lines with heterogeneity interval RM19410 ~ RM5815 had significant genotype effects, while the near-isogenic lines with heterozygous interval RM4923 ~ RM19410 and RM19417 ~ RM204 had no significant variation.After comparison, QHUS6.1 was located in a region of about 64.2kb containing 9 candidate genes between RM19410 and RM19417. This QTL has a strong effect and at the same time controls the silicon content in rice husks, flag leaves and stems. The synergistic alleles are derived from Male parent Miryang 46 was additively inherited.This study laid the foundation for the cloning of qHUS6.1 and provided new ideas for the construction and application of QTL fine mapping material.