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目的:探讨流式细胞仪(FCM)检测膀胱癌患者细胞免疫功能状况的方法,为肿瘤治疗提供参考。方法:用流式细胞仪对72例膀胱癌患者及50例健康对照者人血标本检测其红细胞、中性粒细胞及淋巴细胞上CD35的表达。结果:膀胱癌患者红细胞、中性粒细胞、B淋巴细胞及CD4+T淋巴细胞上CD35的表达比健康人明显降低(P<0.01);而CD8+T淋巴细胞上CD35的表达比健康人明显升高(P<0.01)。结论:建立了FCM检测膀胱癌患者血细胞上CD35分子表达的实验方法。为膀胱癌的诊断、疗效观察及判断预后提供科学而有效的检测方法。
Objective: To investigate the method of flow cytometry (FCM) for detecting cellular immune function in patients with bladder cancer and provide reference for the treatment of cancer. Methods: The expression of CD35 on erythrocytes, neutrophils and lymphocytes of 72 bladder cancer patients and 50 healthy controls were detected by flow cytometry. Results: The expression of CD35 on erythrocytes, neutrophils, B lymphocytes and CD4 + T lymphocytes in bladder cancer patients was significantly lower than that in healthy people (P <0.01), while the expression of CD35 on CD8 + T lymphocytes was significantly higher than that in healthy people Increased (P <0.01). Conclusion: The experimental method of detecting the expression of CD35 on the blood cells of patients with bladder cancer by FCM was established. For the diagnosis of bladder cancer, the efficacy of observation and prognosis to provide scientific and effective detection methods.