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目的探讨红藻氨酸(KA)诱导癫疒间大鼠血清及海马组织中神经元特异性烯醇化酶(NSE)和S100β蛋白(S100β)的变化及其临床意义。方法180只Wistar大鼠随机分为对照组、KA组和卡马西平(CBZ)组,后两组再按癫疒间发作后1h、4h、12h、24h、48h和72h不同时点分为6个亚组。以放射免疫法(RIA)和酶联免疫吸附试验(ELISA)分别测定大鼠血清和海马匀浆液中NSE和S100β的变化。结果癫疒间发作72h内,血清和海马匀浆液中NSE和S100β的含量是一个动态变化过程,且呈同步变化的趋势,在12h时均达到峰值。在4～48h时,KA组和CBZ组的二者含量均明显高于对照组(P<0.05～0.01)。结论癫疒间发作后NSE和S100β的含量升高,二者可作为癫疒间发作后脑组织损伤的一个参考指标。
Objective To investigate the changes and clinical significance of neuron specific enolase (NSE) and S100β protein (S100β) in serum and hippocampus of rats with kainic acid (KA) -induced epilepsy. Methods 180 Wistar rats were randomly divided into control group, KA group and CBZ group. The latter two groups were divided into 6 groups at different time points of 1h, 4h, 12h, 24h, 48h and 72h after epileptic seizure Subgroups. Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) were used to determine the changes of NSE and S100β in serum and hippocampus homogenate respectively. Results The content of NSE and S100β in serum and hippocampus homogenate was a dynamic change within 72 hours of epileptic seizures and showed the trend of synchronous change, reaching the peak value at 12h. At 4 ~ 48h, the contents of KA and CBZ in both groups were significantly higher than those in the control group (P <0.05 ~ 0.01). Conclusion The levels of NSE and S100β increased after the onset of epileptic seizures, both of which may serve as a reference index for the brain damage after epileptic seizures.