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目的探讨人乳头瘤病毒、p16及pRb在宫颈癌的表达及其相关性。方法宫颈上皮内瘤样变(cervicalintraepithelial neoplasia,CIN)Ⅰ型患者108例,CINⅡ型患者50例,CINⅢ型患者35例及宫颈癌患者52例,应用表面等离子体谐振技术、免疫组织化学方法检测人乳头瘤病毒P16及pRb的表达情况。结果从CIN到宫颈癌患者人乳头瘤病毒、P16阳性率呈逐渐增高趋势,pRb阳性率呈逐渐下降趋势;P16表达与pRb表达呈负相关(r=-0.537,P<0.01),人乳头瘤病毒与pRb表达呈负相关(r=-0.513,P<0.05),人乳头瘤病毒与P16表达呈正相关(r=0.815,P<0.05)。结论人乳头瘤病毒感染是宫颈癌发生的主要原因,p16可作为宫颈癌的早期预测指标。
Objective To investigate the expression and correlation of human papillomavirus (HPV), p16 and pRb in cervical cancer. Methods One hundred and eight patients with cervical intraepithelial neoplasia (CIN) Ⅰ type, 50 patients with CIN Ⅱ type, 35 patients with CIN Ⅲ type and 52 patients with cervical cancer were examined by surface plasmon resonance and immunohistochemistry Papillomavirus P16 and pRb expression. Results The positive rate of P16 was gradually increased and the positive rate of pRb was gradually decreased from CIN to HPV in human cervical cancer patients. The expression of P16 was negatively correlated with the expression of pRb (r = -0.537, P <0.01) There was a negative correlation between the expression of pRb and the virus (r = -0.513, P <0.05). The positive correlation was found between HPV and P16 (r = 0.815, P <0.05). Conclusion Human papillomavirus (HPV) infection is the main cause of cervical cancer, and p16 may be used as an early predictor of cervical cancer.