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采用特异、敏感的RT -PCR技术 ,检测来自 2 0例乳腺癌病人的 10 8个HE染色阴性的腋窝淋巴结MUC1mRNA的表达 ,结果MUC11mRNA在 10 8个HE染色阴性淋巴结中阳性检出率为 66% .提示 :这些淋巴结中存在肿瘤隐蔽淋巴结转移 ,这种转移可能是一个或数个肿瘤细胞的转移 ,以此来提高微转移诊断率 ,可指导术后治疗 ,延长患者生存期 .结果表明 :MUC11mRNA的RT -PCR分析法可用于乳癌淋巴结微转移诊断中 ,具有很高的临床使用价值 .
The specific and sensitive RT-PCR technique was used to detect the expression of 108 HE-negative MVD1 mRNA in axillary lymph nodes from 20 breast cancer patients. The positive rate of MUC11 mRNA in 106 HE-negative lymph nodes was 66% .There is a hidden tumor metastasis in these lymph nodes.The metastasis may be the metastasis of one or several tumor cells in order to improve the diagnostic rate of micrometastasis and guide the postoperative treatment to prolong the survival of patients.The results showed that MUC11mRNA The RT-PCR assay can be used in the diagnosis of lymph node micrometastases in breast cancer with high clinical value.