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目的建立UPLC-Q-TOF/MS同时测定强力天麻杜仲胶囊中天麻素、京尼平苷酸、松脂醇二葡萄苷、苯甲酰新乌头原碱及蛇床子素含量的方法。方法采用Waters ACQUITY UPLC,色谱柱为CORTECSTM C18柱(4.6 mm×150 mm,2.7μm),流动相为乙腈(A)-0.1%甲酸水溶液(B)梯度洗脱,电喷雾离子源(ESI),正负离子模式,质量扫描范围为50~1 200 Da。结果 5种被测成分在线性范围内均具有良好的线性关系(r2≥0.997 4),平均回收率在99.533%~107.525%,RSD≤2.56%。结论应用UPLC-Q-TOF/MS法分离效果及重复性好,且快速、简便,可作为强力天麻杜仲胶囊的质量控制方法。
OBJECTIVE To establish a method for the simultaneous determination of gastrodin, geniposidic acid, pinoresinol, benzoyl-aconitine and osthole in Qiangli Tianma Eucommiae by UPLC-Q-TOF / MS. Methods Waters ACQUITY UPLC was used with CORTECSTM C18 column (4.6 mm × 150 mm, 2.7 μm) as mobile phase and gradient elution with acetonitrile (A) - 0.1% formic acid as solution (B). Electrospray ionization (ESI) Positive and negative ion mode, the quality of scan range of 50 ~ 1 200 Da. Results The five analytes had a good linearity within the linear range (r2 ≥ 0.997 4). The average recoveries ranged from 99.533% to 107.525% with RSD ≤ 2.56%. Conclusion The UPLC-Q-TOF / MS method has the advantages of good separation and repeatability, rapid and simple, and can be used as a quality control method for the powerful Tianma Eucommia capsule.