【摘 要】
：
The short neuropeptide F(sNPF)and NPF receptor(NPFR)genes play impor-tant roles in many physiological processes.However,information on the survival-related functions of sNPF and NPFR under different stress conditions is lacking in aphids.In this study,we
【机 构】
：
State Key Laboratory of Crop Stress Biology for Arid Areas,Key Laboratory of Crop Pest Integrated Pe
论文部分内容阅读
The short neuropeptide F(sNPF)and NPF receptor(NPFR)genes play impor-tant roles in many physiological processes.However,information on the survival-related functions of sNPF and NPFR under different stress conditions is lacking in aphids.In this study,we cloned sNPF and NPFR,and investigated the expression levels of these genes in different developmental stages,wing morphs,and stress conditions of the bird cherry-oat aphid(Rhopalosiphum padi L.),an important agricultural pest.The sNPF and NPFR transcript levels varied among developmental stages,and their expression levels in alate females were significantly higher than those in apterous females.In addition,starvation resulted in significantly increased sNPF expression,which then recovered after refeeding.Heat stress and insecticides significantly affected transcription of both genes.sNPF and NPFR knockdown in R.padi using RNA interference revealed optimal interference effi-ciency at 48 h post-injection.sNPF knockdown significantly decreased adult longevity,15-d fecundity,and food intake.Additionally,mortality under starvation,insecticides,and heat stress conditions was significantly higher after injection with double-stranded sNPF in R.padi.NPFR knockdown significantly affected food intake and starvation resistance in R.padi.These results strongly indicate that sNPF plays vital roles in food intake,longevity,and reproduction in R.padi,and it can significantly affect the pest\'s response to stress conditions.
其他文献
Dear Editor,rnAnopheles sinensis is the primary vector of vivax malaria in China.Given the risks posed by An.sinen-sis,many methods have been used to control this vec-tor,most commonly chemical prevention.However,the extensive use of insecticides has subj
阅读在科学学习中发挥着重要的作用,中考物理试题可以引领科学阅读.我们选取了近六年的北京市中考物理真题,对其中的科普阅读题进行分析,研究其命题特点,提出了培养学生科学阅读素养的途径.
The ladybird Harmonia axyridis is an insect that exhibits pupal attachment to plants,which facilitates development and environmental adaptation.The cremaster is highly specialized for this behavior.However,the underlying molecular regulation of the cremas
The Asian citrus psyllid,Diaphorina citri is the principal vector of huang-longbing,which transmits Candidatus Liberibacter asiaticus.Trehalase is a key enzyme involved in trehalose hydrolysis and plays an important role in insect growth and develop-ment.
考试评价是教学的有机组成部分,也是让核心素养落地的最直接、最重要的保障.本文以2019年4月浙江省物理选考试题第18题为例,从学科核心素养的水平层次、试题命制的立意、情境和知识维度等方面,论述物理高考试题在评价目标、内容设计等方面以问题解决促进物理学科核心素养的达成所呈现的特征和特色.
通过对近十年全国及各地高考(选考)物理试题的筛选和分析,例举了具有科学论证素养的部分试题,总结归纳了此类试题的特点,并对教学中如何培养学生的科学论证素养提出了建议.
本文以“简谐运动”一课的教学设计为例,阐述了“基于学科方法理解”的教学模式探索.本模式通过创设丰富的情境,打破学科壁垒,探寻方法联结,促进学生对学科方法的理解,发展学生的科学思维能力,将学生核心素养的培养落到实处.
对于刚体转动一般采用角量来描述,而角量是相对于某个转轴而言的.选择合适的转轴可以简化问题的求解.本文以第37届全国中学生物理竞赛复赛试题第二题为例,分析了连滚带滑问题的运动学和动力学规律,给出了两种求解方法.第一种方法针对运动的不同阶段分析求解,便于理解运动过程的物理特征;另一种方法通过选择合适的转轴利用角动量守恒定律,使得求解过程大大简化.
能量观是物理核心素养的重要观念,利用能量观,对比两种电磁感应的模型,理解和处理电磁感应中的几种功率,有利于加强学生物理观念的培养,发展学生逻辑推理及信息辨析的能力.
《普通高中物理课程标准(2017年版)》规定了 21个学生必做实验,新出版的人教版高中物理必修教材编排了学生必做实验.详细分析发现人教版高中物理必修教材学生必做实验的编排具有几个特征:实验的结构清晰,思路明确;实验由问题驱动,步步推进;实验呈半开放性,合理探究;实验重方法教学,层层深入.