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目的:通过研究腺嘌呤对醛固酮合成酶途径的影响,探讨其导致“肾虚多尿”的机制所在。方法:利用酶联免疫分析法分别检测正常组、腺嘌呤组动物血中皮质酮(Cort)、醛固酮(ALD)的含量;并采用RT-PCR的方法检测各组动物肾脏CYP11B2 mRNA的表达。结果:与正常组比较,腺嘌呤可明显减少大鼠血中Cort,ALD含量,并下调模型大鼠肾脏CYP11B2mRNA的表达。结论:腺嘌呤肾虚多尿大鼠血中Cort,ALD含量减少,肾脏CYP11B2 mRNA的表达下调,影响醛固酮合成酶途径,从而减少ALD的合成,导致水液代谢异常引发多尿,本实验研究结果从醛固酮合成酶的角度阐明了腺嘌呤导致“肾虚多尿”的作用机制。
Objective: To investigate the effect of adenine on aldosterone synthase pathway and to explore the mechanism of “kidney-kidney-polyuria”. Methods: The contents of corticosterone, aldosterone (ALD) in normal and adenine groups were detected by enzyme-linked immunosorbent assay. The expression of CYP11B2 mRNA in kidney of each group was detected by RT-PCR. Results: Compared with the normal group, adenine can significantly reduce the Cort and ALD levels in the blood and decrease the expression of CYP11B2 mRNA in the kidney of model rats. CONCLUSION: Cort and ALD levels in the blood of adenine-induced renal polyuria are decreased, and the expression of CYP11B2 mRNA in the kidney is down-regulated, which affects the pathway of aldosterone synthase, thereby reducing the synthesis of ALD, resulting in abnormal urine-water metabolism. The results of this study The aldosterone synthase perspective clarifies the mechanism of action of adenine resulting in “kidney-polyuria”.