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目的观察乳果糖溶液对实验动物的通便功能。方法使用雄性ICR小鼠,按体重均衡分为5组――阴性对照、模型对照和3个剂量组,剂量组分别经口给予乳果糖溶液0.2mL/kg BW、2.0mL/kg BW和6.0mL/kg BW,0.0mL/kg BW组(包括阴性对照组和模型对照组)以水代替受试物。7d后,经口给予复方地芬诺酯造模,实验中,记录每只小鼠小肠墨汁推进率、首粒排黑便时间、5小时内排便粒数和排便重量。结果模型对照组与阴性对照组相比,差异具有极显著性(P<0.01),造模成功。在2.0mL/kg BW和6.0mL/kg BW组与模型对照组相比,提高小肠墨汁推进率,缩短首粒排便时间,差异均有统计学意义(P<0.01),增加小鼠排便粒数,差异有统计学意义(P<0.05)。对小鼠的体重增长和小鼠排便重量无不良影响,实验期间各组小鼠的粪便均为椭圆形粒状干便,无腹泻现象发生。结论乳果糖溶液对小鼠具有明显通便功能,且对机体健康无不良影响。
Objective To observe the laxative effect of lactulose solution on experimental animals. Methods The male ICR mice were divided into 5 groups according to body weight: negative control, model control and 3 dose groups. The dose groups were orally administered lactulose solution 0.2mL / kg BW, 2.0mL / kg BW and 6.0mL / kg BW, 0.0 mL / kg BW group (including the negative control group and model control group) with water instead of the test substance. After 7 days, the compound diphenoxylate was given orally. In the experiment, the rate of advancement of small intestine ink in each mouse, the first pellet discharge time, the number of defecation granules in 5 hours and the weight of defecation were recorded. Results The difference between the model control group and the negative control group was significant (P <0.01), and the model was successful. In 2.0 mL / kg BW and 6.0 mL / kg BW group, compared with the model control group, the promoting rate of small intestine ink and the first defecation time were shortened, the differences were statistically significant (P <0.01) , The difference was statistically significant (P <0.05). There was no adverse effect on body weight gain and defecation weight of mice. During the experiment, all the mice ’s stools were oval granular dry stool without diarrhea. Conclusion Lactulose solution has obvious cathartic effect on mice and has no adverse effect on the health of the mice.