目的通过对初断乳大鼠的高钙干预及成年后的高脂干预,测定大鼠骨骼肌中解偶联蛋白2(UCP2)mRNA的表达水平,探讨生命早期高钙摄入对成年期肥胖形成的影响。方法初断乳雄性Wistar大鼠120只随机分为正常饲料组和高钙饲料低(2%)、中(2.5%)、高剂量(3%)组。高钙干预4周后检测血脂。随后所有大鼠进行为期3周的基础饲料喂养。于第7周末取血检测血脂。将正常饲料组成年大鼠随机分为正常对照组和肥胖诱导组,肥胖诱导组及3个高钙组均喂以高脂饲料,肥胖诱导8周后处死所有大鼠取血检测血脂并冻存骨骼肌RT-PCR法半定量检测UCP 2基因表达水平。结果高脂干预后3个高钙组的体重增长量均低于肥胖诱导组,低剂量和高剂量组的体重增长量与正常对照组没有差别;高剂量组的血清甘油三酯含量明显低于肥胖诱导组,与正常对照组一致;肥胖诱导组和高剂量组的UCP 2基因表达水平明显低于正常对照组、低剂量和中剂量组;中剂量高钙组的表达水平明显高于正常对照组。结论初断乳大鼠的高钙喂养能够持续发挥作用,使其在成年后的高脂饲料喂养期间,UCP2基因表达增强,血脂紊乱得以改善。 OBJECTIVE: To determine the expression level of uncoupling protein-2 (UCP2) mRNA in rat skeletal muscle by high-calcium intervention in early-weaned rats and high fat diet in adult rats, and to investigate the effect of high calcium intake on early obesity The formation of the impact. Methods 120 severed male Wistar rats were randomly divided into normal diet group and high calcium diet (2%), middle (2.5%) and high dose (3%) groups. After 4 weeks of high calcium intervention, blood lipids were measured. All rats were subsequently fed a basal diet for 3 weeks. At the end of the seventh weekend blood test for lipid. The rats of normal diet were randomly divided into normal control group and obesity induction group. Fat-induced group and high calcium group were fed with high-fat diet. After 8 weeks of obesity induction, all rats were sacrificed to measure blood lipids and cryopreserved Skeletal muscle RT-PCR semi-quantitative detection of UCP 2 gene expression levels. Results After high-fat diet, the body weight gain of three high-calcium groups was lower than that of the obesity-induced group. There was no difference between the high-fat group and the normal control group. The high-dose group had significantly lower serum triglyceride Obesity-induced group, consistent with the normal control group; obesity-induced group and high-dose group UCP 2 gene expression was significantly lower than the normal control group, low dose and middle dose group; medium dose of high calcium group expression was significantly higher than the normal control group. Conclusion The high calcium diet in weaned rats can continue to play a role in enhancing the expression of UCP2 gene and improving dyslipidemia during the adult high fat diet.