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目的:通过大鼠短暂性大脑中动脉阻塞后梗死体积的测量和神经功能评分,评价改良大鼠大脑中动脉阻塞模型的可靠性。方法:分别使用包被有多聚-L-赖氨酸(0·1%)尼龙线和普通尼龙线,线栓法制作大鼠大脑中动脉阻塞模型,缺血2h后再灌注,分别在再灌注后3、24、48和72h联合应用八分法和姿势反射评价运动神经功能,3d后处死大鼠,测定梗死体积并进行比较。结果:运动神经功能评分结果与梗死体积成线性关系,多聚-L-赖氨酸包被线组制作模型的成功率高于普通线组,且梗死体积比普通线组大。结论:神经功能评分能很好的反映脑梗死的严重程度;采用多聚-L-赖氨酸包被的尼龙线能使大鼠大脑中动脉阻塞模型更可靠,成功率高,梗死体积和部位变异性小。
OBJECTIVE: To evaluate the reliability of a modified middle cerebral artery occlusion model in rats by measuring infarct volume and neurological function after transient middle cerebral artery occlusion in rats. Methods: The model of middle cerebral artery occlusion in rats was made by using the poly-L-lysine (0.1%) nylon thread and ordinary nylon thread respectively. After ischemia for 2 hours, the middle cerebral artery occlusion model was established. At 3, 24, 48 and 72h after perfusion, motor neurological function was evaluated by using the dichotomous method and postural reflex. The rats were sacrificed after 3d and infarct volume was measured and compared. Results: The score of motor neurological function was linear with infarct volume. The success rate of poly-L-lysine coated line group was higher than that of normal line group, and infarct volume was larger than that of normal line group. Conclusion: Nerve function score can reflect the severity of cerebral infarction. Nylon coated with poly-L-lysine can make middle cerebral artery occlusion model of rat more reliable, high success rate, infarct size and location Variability is small.