目的:探讨产前筛查及产前诊断在临床的应用及意义。方法:应用时间分辨荧光免疫法检测血清学三联指标(AFP+F-β-HCG+uE3)水平,Life-Cycle软件计算风险值;对产前筛查孕妇随访结果进行整理和分析。结果:15 486例孕妇中,血清学筛查高风险860例,筛查阳性率为5.55%;年龄高风险712例。高风险孕妇中575例(36.58%)选择产前诊断,羊水诊断检出21-三体8例、18-三体1例,产前超声诊断检出无脑儿1例。高风险孕妇不良妊娠结局发生率(5.36%)与低风险孕妇(2.02%)相比,差异有统计学意义(P<0.01);多种高风险孕妇不良妊娠结局发生率(26.92%)与仅21-三体或18-三体高风险孕妇(7.74%)相比,差异有统计学意义(P<0.01)。结论:产前筛查结果是预测染色体异常胎儿和不良妊娠结局的重要指标,结合产前诊断能有效防止出生缺陷发生;正确有效的随访对产前工作有重要意义。 Objective: To explore the clinical application and significance of prenatal screening and prenatal diagnosis. Methods: The level of serum triple index (AFP + F-β-HCG + uE3) was detected by time-resolved fluorescence immunoassay. Risk-value was calculated by Life-Cycle software. The follow-up results of prenatal screening pregnant women were collected and analyzed. Results: Among 15 486 pregnant women, 860 were serologically risk-tested, with a positive screening rate of 5.55% and a high-risk age of 712. Among 575 high-risk pregnant women (36.58%), prenatal diagnosis was selected. In amniotic fluid diagnosis, 8 cases of 21-trisomy, 1 case of 18-trisomy, 1 case of anencephaly were detected by prenatal ultrasound. The incidence of adverse pregnancy outcomes in high-risk pregnant women (5.36%) was significantly lower than that in low-risk pregnant women (2.02%) (P <0.01). The incidence of adverse pregnancy outcomes in multiple high-risk pregnant women (26.92% 21- trisomy or 18- trisomy high-risk pregnant women (7.74%), the difference was statistically significant (P <0.01). Conclusion: Prenatal screening is an important index for predicting fetal and abnormal pregnancy outcomes. Prenatal diagnosis can effectively prevent birth defects. Correct and effective follow-up is of great importance to prenatal work.