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为了观察Toll样受体1(TLR1)及FasL在大鼠哮喘中的作用及布地奈德(BUD)对其的影响,采用大鼠哮喘模型,随机分成正常对照组、哮喘组、布地奈德治疗组为研究对象,取外周血,流式细胞仪检测其淋巴细胞上TLR1和FasL的表达水平,右肺行病理学检查。结果发现:哮喘组外周血淋巴细胞TLR1、FasL表达水平均显著高于对照组(P<0.01),且与BUD组比较TLR1、FasL表达有显著性差异(P<0.01)。流式细胞散点图提示哮喘组淋巴细胞TLR1呈高表达。在哮喘组和BUD组以及正常对照组外周血淋巴细胞FasL表达均较低,但是,在哮喘组仍明显高于正常对照组(P<0.01)。提示:淋巴细胞上TLR1和FasL的表达水平在哮喘时增加,TLR1、FasL参与了哮喘的发病机制,TLR1在哮喘的气道慢性炎症的发生和发展中起重要作用,而哮喘组存在淋巴细胞凋亡下降,可能是哮喘气道慢性炎症持续存在的原因。作者认为BUD吸入有可能通过调节淋巴细胞凋亡的平衡来治疗哮喘。
In order to observe the role of Toll-like receptor 1 (TLR1) and FasL in asthmatic rats and the effect of budesonide (BUD) on them, the rat asthma model was randomly divided into normal control group, asthma group and budesonide Group as the research object. Peripheral blood and peripheral blood were collected. The expression of TLR1 and FasL on lymphocytes were detected by flow cytometry. Pathology examination was performed on the right lung. The results showed that the expressions of TLR1 and FasL in peripheral blood lymphocytes in asthma group were significantly higher than those in control group (P <0.01), and the expressions of TLR1 and FasL in asthmatic group were significantly different from those in BUD group (P <0.01). Flow cytometry scatter plots suggest that the expression of TLR1 in asthmatic group was high. The expression of FasL in peripheral blood lymphocytes in asthma group, BUD group and normal control group was lower than that in normal control group (P <0.01). It is suggested that the expression of TLR1 and FasL on lymphocytes is increased in asthma, TLR1 and FasL are involved in the pathogenesis of asthma, TLR1 plays an important role in the occurrence and development of chronic airway inflammation in asthma, while lymphocyte apoptosis Falling death may be the reason for the persistence of chronic airway inflammation in asthma. Authors believe that BUD inhalation may be able to treat asthma by regulating the balance of lymphocyte apoptosis.