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目的 为揭示脑内参与神经免疫调节过程的部位和核团。方法 大鼠腹腔内给予细菌内毒素脂多糖 (LPS)或葡萄球菌肠毒素B (SEB) ,用免疫组织化学方法观察了Ⅰ型IL 1受体在脑内表达的变化。结果 Ⅰ型IL 1受体在正常成年大鼠脑内有广泛的表达 ,隔区、视前内侧区、新皮质、海马、下丘脑室旁核、视上核、下丘脑腹内侧核、弓状核和正中隆起等部位有较多Ⅰ型IL 1受体阳性细胞。与生理盐水对照组和非免疫应激对照组 (强迫游泳 )比较 ,LPS或SEB腹腔注射后大鼠下丘脑室旁核和视上核中表达Ⅰ型IL 1受体的细胞数量显著增加 ,染色加深 (P <0 0 5 )。阳性细胞的胞浆染色面积增大 ,突起染色的长度延长。结论 下丘脑室旁核和视上核在神经免疫调节过程中可能具有重要的作用。
The purpose is to reveal the parts of the brain and nuclei that participate in the neural immunomodulation process. Methods LPS or SEB were intraperitoneally administered to rats. The expression of IL - 1 receptor in brain was observed by immunohistochemistry. Results The type 1 IL 1 receptor was widely expressed in the brain of normal adult rats. The septal area, the anteromedial medial area, the neocortex, the hippocampus, the paraventricular nucleus of the hypothalamus, the supraoptic nucleus, the ventromedial nucleus of the hypothalamus, There were more type I IL-1 receptor positive cells in the nucleus and mid-limb. Compared with saline control group and non-immune stress control group (forced swimming), the number of type I IL-1 receptors in hypothalamic paraventricular nucleus and supraoptic nucleus significantly increased after intraperitoneal injection of LPS or SEB. Deepened (P <0.05). Positive cytoplasm stained area increased, the length of the protuberant staining prolonged. Conclusion The hypothalamic paraventricular nucleus and supraoptic nucleus may play an important role in the regulation of neuroimmunity.