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菠萝粉蚧Dysmicoccus brevipes(Cockerell)和新菠萝灰粉蚧Dysmicoccus neobrevipes(Beardsley)是菠萝等热带水果上的重要害虫,是我国进出境水果贸易中均需要检疫处理的对象。为了建立适合菠萝等水果上这两种粉蚧的检疫处理技术,本研究采用80、130、180、230和280 Gyγ-射线辐照处理了两种粉蚧的若虫和成虫。结果表明,两种粉蚧对γ射线的剂量反应基本一致,表现为随虫龄增大对辐照的耐受性增强,以雌成虫为最强。采用230Gy辐照若虫,两种粉蚧各若虫均不产生F1代活虫体,并且菠萝粉蚧1龄若虫在该剂量下不能发育至雌成虫。以230 Gy辐照成虫,两种粉蚧均产生F1代雌成虫,但不产生F2代。以240 Gy剂量大规模辐照处理3龄若虫和雌成虫,表明该剂量足以阻止两种粉蚧繁殖,该剂量可作为这两种粉蚧辐照处理的参考指标。
Dysmicoccus brevipes (Cockerell) and Dysmicoccus neobrevipes (Beardsley) are important pests on tropical fruits such as pineapple and are the objects of quarantine treatment in the trade of inbound and outbound fruits in China. In order to establish a quarantine treatment technique suitable for the two mealybugs, such as pineapple, the nymphs and adults of two mealybugs were treated with γ-ray irradiation of 80, 130, 180, 230 and 280 Gy. The results showed that the dose response of the two mealybugs to γ-rays was basically the same, showing the enhanced tolerance to irradiation with increasing insects ages, and the female adult was the strongest. Using 230Gy irradiated nymphs, each of the two mealybugs did not produce F1 live worms, and the first instar nymphs of P. gratensis did not develop to female adults at this dose. Adults were irradiated with 230 Gy, and both mealybugs produced F1 generation female but did not produce F2. Large-scale irradiation with 240 Gy doses of 3rd instar nymphs and adult worms indicated that this dose was sufficient to prevent the two mealybugs from multiplying, which could be used as a reference for the irradiation of these two mealybugs.