解决斑叶秋海棠的种质保存和种苗保留问题,我们用斑叶秋海棠叶片进行组织培养,并配比多种植物激素组合繁育试管苗,已成功地诱导发生了大量种苗和少量盆栽试管苗。(一)材料和方法切取斑叶秋海棠叶片,用清水多次洗涤,置无 To solve the germplasm conservation and seedling preservation of the mutant Begonia, we used tissue culture of leaflet Begonia and combined it with a variety of plant hormones to breed the plantlets successfully. A large number of seedlings and a few potted plantlets have been successfully induced . (A) Materials and methods Cut off the leaves of Begonia Begonia, washed repeatedly with water, set no