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脱氧核糖核酸酶I(DeoxyribonucleaseI,DNase I)是最早发现的一种特异性核酸内切酶,是一种38KDa的糖蛋白,作用于双链DNA的磷酸二酯键,产生带有5′-磷酸、3′一羟基末端的寡核苷酸,其活性依赖于Mg2+和Ca2+等二价阳离子的存在。最初在消化道中被发现,一度认为它仅是一种消化酶,作用是为体内核苷酸的补救合成途径提供寡核苷。本文应用PCR-LDR法检测DNase I基因A2317G位点的基因型,分析汉、维族急性心肌梗塞患者的基因多态性特点。
Deoxyribonuclease I (DNase I) was one of the earliest found specific endonucleases, a 38 kDa glycoprotein that acts on the phosphodiester bond of double-stranded DNA to create a DNA fragment with 5’-phosphate , 3 ’-hydroxyl-terminated oligonucleotide whose activity depends on the presence of divalent cations such as Mg2 + and Ca2 +. Originally found in the digestive tract, it was once thought that it was only a digestive enzyme that served to provide oligonucleosides for the body’s nucleotide synthesis pathway. In this study, PCR-LDR method was used to detect the genotype of DNase I gene A2317G, and to analyze the gene polymorphism of Han and Uygur patients with AMI.