[目的]为开展桔小实蝇检疫防控技术研究提供技术基础。[方法]利用养虫室、养虫笼等设施,采用人工饲养和果实培养等方法建立和维持桔小实蝇种群并进行持续的室内生物学观察;在太湖东山地区建立隔离网室,配备温湿度记录装置,栽种各类寄主植物,每天观察桔小实蝇的生活习性。[结果]通过实验室饲养观察,掌握了桔小实蝇成虫、幼虫、蛹和卵的生活习性;通过土壤深度对蛹羽化率影响的试验可知将土层深度加大到40cm仍有成虫出现,且羽化率为80%;通过室内趋向性试验,发现桔小实蝇对苏州地产水果的选择性从高到低排序为柑橘、枣、石榴、桃、橙、梨、柿子。而野外调查表明,果树品种根据桔小实蝇为害率从高到低排序为柑橘、枣、石榴、梨、柿子。在本地柑橘品种中,其为害率从高到低排序为洞庭蜜橘、早橘、温柑、黄皮、早红、料红。[结论]建立了桔小实蝇人工饲养设施,为开展桔小实蝇生物学特性观察和生物学试验奠定了基础。 [Objective] The research aimed to provide the technical basis for the research on quarantine prevention and control technology of Tricholoma matsutake. [Method] With the help of rearing house, rearing cages and other facilities, artificial breeding and fruit cultivation methods were used to establish and maintain the population of the orange fruit fly and to conduct continuous indoor biological observation. An isolation house was set up in Dongshan district of Taihu Lake, Humidity recording device, planting all kinds of host plants, observe the daily life of orange fruit fly. [Result] The living habits of adults, larvae, pupae and eggs of Bombyx mori was grasped by laboratory observation. By the test of the effect of soil depth on the emergence rate of pupae, it was found that adults were still present when the soil depth was increased to 40cm, And the eclosion rate was 80%. According to indoor trend test, it was found that the selectivity of fruit flies from Suzhou to Suzhou was orange, date, pomegranate, peach, orange, pear and persimmon. The field survey showed that the fruit trees were classified as citrus, jujube, pomegranate, pear and persimmons according to their damage rates from high to low. In the local citrus varieties, the damage rate ranked from high to low as Dongting tangerine, early orange, warm orange, yellow, red early, red. [Conclusion] The artificial feeding facility of orange fruit fly was established and laid the foundation for the observation of biological characteristics and biological experiment of fruit fly.