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以链球菌溶血素“O”(SLO)作抗原,与病人血清在琼脂中作双向扩散试验,能形成单一的白色沉淀线,表示标本中含有与SLO相应的特异性抗体。将待检标本作不同比例的稀释,可以测知病人血中ASO滴度的高低。本文报道应用这一方法的结果。 一、材料 (一)SLO系上海市洋泾人民医院生产。 (二)缓冲液:称取Tris 1.2 g,Nacl 5.8 g,EDTA 0.4和叠氮钠300mg,溶于蒸馏水并稀释到1000ml。 二、方法 (一)用上述缓冲液配制1％优质琼脂凝胶,加
With streptolysin “O” (SLO) as antigen, and patient serum agar for two-way diffusion test, a single white precipitate line can be formed, indicating that the specimen contains SLO-specific antibodies. The specimens to be seized for different dilution ratio, you can measure the level of blood in patients with ASO titers. This article reports the results of applying this method. First, the material (a) SLO Shanghai Yang Jing People’s Hospital production. (B) Buffer: Weigh Tris 1.2 g, Nacl 5.8 g, EDTA 0.4 and sodium azide 300mg, dissolved in distilled water and diluted to 1000ml. Second, the method (a) with the above buffer formulated 1% quality agar gel, add