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[目的 ]探讨尿液中血吸虫循环抗原和抗体检测对日本血吸虫病的诊断价值。 [方法 ]用单克隆抗体夹心 ELISA法检测日本血吸虫病患者尿液中循环抗原 ,间接ELISA检测尿液中特异性抗体。 [结果 ]10例急性血吸虫病和 6 1例慢性血吸虫病患者尿液中循环抗原的阳性率分别为 6 0 %和 40 % ,特异性抗体的阳性率分别为 80 %和 6 1 7%。两者联合检测的总阳性率分别为 10 0 %和 71 7%。 10 0例健康对照者尿液中仅 3%出现假阳性。 [结论 ]检测尿液中日本血吸虫循环抗原和特异性抗体简便、实用 ,为一种非损伤性的血吸虫病诊断方法。
[Objective] To investigate the diagnostic value of urinary schistosoma circulating antigen and antibody test on schistosomiasis japonica. [Method] Monoclonal antibody sandwich ELISA was used to detect urinary circulating antigens in patients with schistosomiasis japonica and indirect ELISA was used to detect specific antibodies in urine. [Results] The positive rates of urinary circulating antigens in 10 cases of acute schistosomiasis and 61 cases of chronic schistosomiasis were 60% and 40% respectively, and the positive rates of specific antibodies were 80% and 61.7% respectively. The combined positive rates of the two assays were 100% and 71%, respectively. 10% of healthy controls urine only 3% false positives. [Conclusion] It is a simple and practical method to detect circulating antigen and specific antibody of Schistosoma japonicum in urine. It is a noninvasive diagnostic method for schistosomiasis.