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目的通过对多发性硬化患者外周血单个核细胞中环孢素 A受体 m RNA表达的研究 ,为临床采用环孢素 A辅助治疗该病提供一定的依据。方法采用逆转录 PCR方法 ,结果经凝胶图像分析。结果患者组即多发性硬化患者外周血单个核细胞存在有 Cy P m RNA的表达 ,与对照组相比较降低 ,尚无明显统计学差异 (P>0 .0 1)。结论多发性硬化患者外周血单个核细胞中存在有 Cy P,Cs A与细胞内的 Cy P结合后是否产生生物活性还与其蛋白质的构象等有关
Objective To study the expression of cyclosporin A receptor m RNA in peripheral blood mononuclear cells in patients with multiple sclerosis and to provide a basis for clinical use of ciclosporin A adjuvant therapy. Methods Reverse transcription PCR was used and the results were analyzed by gel images. Results The expression of Cy P m RNA in peripheral blood mononuclear cells of patients with multiple sclerosis was significantly lower than that of the control group (P 0. 01). Conclusions The presence of Cy P in peripheral blood mononuclear cells from patients with multiple sclerosis and the combination of Cs A and intracellular Cy P have biological activities related to their protein conformation