用~60Co-γ射线,甲基磺酸乙酯(EMS)处理组培甘蔗愈伤组织,可提高再生植株的变异机率,处理愈伤组织再生植株性状:株高、茎径、锤度等明显偏离正常的正态分布,差异达1%显著标准,EMS处理较~60Co-γ射线变化大。连续试验观察表明:一些变异性状具有遗传性。用~60Co-γ射线或甲基磺酸乙酯处理甘蔗愈伤组织可作为一种甘蔗体细胞诱变育种手段加以利用。 The tissue culture of sugarcane callus with ~ 60Co-γ ray and ethyl methylsulfonate (EMS) could increase the mutation rate of regenerated plants, and the regenerated plant traits of callus were treated by EMS: the plant height, stem diameter and brix were obvious Deviated from the normal distribution, the difference reached 1% significant standard, EMS treatment than ~ 60Co-gamma ray changes. Continuous experimental observations show that some of the variability traits are hereditary. Treatment of sugarcane callus with ~ 60Co-gamma rays or methyl methanesulfonate can be used as a means of mutation breeding of sugarcane somatic cells.