目的:探讨CYP17A1介导的DNA去甲基化与胶质瘤细胞增殖、侵袭和转移的关系。方法:将2019年6月收集的组织细胞样本通过聚合酶链式反应(PCR)实验，蛋白质印迹法(Western blot)实验对细胞中CYP17A1 Mrna及蛋白表达量进行测定；利用甲基化检测(MSP)法检测CYP17A1基因在的甲基化状态；通过噻唑蓝(MTT)比色法及流式细胞仪对细胞增殖情况进行测定；通过细胞侵袭实验对细胞侵袭及转移情况进行测定。计数资料使用n χ2检验或Fisher精确概率法。n 结果:模型组、实验组与正常组中CYP17A1基因mRNA的相对表达量差异有统计学意义(n F＝12.541，n P<0.05)。实验组与癌模型组比较，CYP17A1基因mRNA的相对表达量显著下降，差异有统计学意义(n t＝12.769，n P<0.05)；免疫组织化学检测CYP17A1蛋白的表达，结果表明，CYP17 A1蛋白在胶质瘤中表达量(0.621±0.027)显著高于正常组(0.019±0.003)，差异有统计学意义(n t＝49.551，n P<0.05)；CYP17A1基因甲基化状态的分析结果表明，CYP17A1基因在正常细胞中未检测出，在模型组中CYP17A1基因甲基化发生率为89.03%，实验组中甲基化发生率为43.93%明显低于模型组，差异有统计学意义(n χ2＝4.021，n P<0.05)；MTT生长曲线结果表明，DHEA结合TMZ预处理能显著抑制细胞增殖速率(n P<0.05)。在平板克隆实验中，CYP17A1在模型组和实验组中克隆个数分别为(78.09±10.21)、(38.97±11.32)个，且实验组中的克隆个数显著低于模型组，差异有统计学意义(n t＝5.738，n P<0.05)；模型组的迁移能力明显高于对照组，实验组细胞的侵袭率33.33%，明显低于模型组的88.89%，差异有统计学意义(n χ2＝5.844，n P<0.05)。n 结论:CYP17A1诱导DNA去甲基化可抑制胶质瘤细胞的增殖、侵袭和转移。“,”Objective:To investigate the relationship between CYP17A1-mediated DNA demethylation and glioma cell proliferation, invasion and metastasis.Methods:Tissue cell samples collected in June 2019, the expression of CYP17A1 mRNA and protein in cells was determined by PCR and Western blotting, the methylation status of CYP17A1 gene was detected by MSP, the proliferation of cells was measured by MTT and flow cytometry, and the invasion and metastasis of cells were determined by cell invasion experiment.Results:There was significant difference in the relative expression of CYP17A1 gene in model group, experimental group and normal group (n F=12.541, n P<0.05). The relative expression of CYP17A1 gene in experimental group was significantly lower than that in cancer model group, the difference was statistically significant (n t=12.769, n P<0.05). The expression of CYP17A1 protein was detected by immunohistochemistry. The results showed that the expression of CYP17A1 protein in glioma was 0.621±0.027, which was significantly higher than that in normal group of 0.019±0.003, the difference was statistically significant (n t=12.769, n P<0.05). The methylation status of CYP17A1 gene showed that CYP17A1 gene was normal. The methylation rate of CYP17A1 gene was 89.03% in model group and 43.93% in experimental group, which was significantly lower than that in model group, the difference was statistically significant (n χ2=4.021, n P<0.05). MTT growth curve showed that DHEA combined with TMZ pretreatment could significantly inhibit cell proliferation rate (n P<0.05). The number of CYP17A1 clones in the model group and the experimental group were (78.09±10.21) and (38.97±11.32), respectively, and the number of CYP17A1 clones in the experimental group was significantly lower than that in the model group, the difference was statistically significant (n t=5.738, n P<0.05); the migration ability of the model group was significantly higher than that of the control group (n P<0.05); the invasion rate of cells in the experimental group was significantly lower than that in the model group, the difference was statistically significant (n χ2=5.844, n P<0.05).n Conclusion:CYP17A1 DNA demethylation can inhibit the proliferation, invasion and metastasis of glioma cells.