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目的:了解沈阳地区腹泻患者副溶血弧菌的感染情况。方法:本地医院送检的腹泻病人标本,经分离培养、生化鉴定、血清分型以及PCR技术检测毒力基因。结果:193份腹泻病人的标本中,检出副溶血弧菌43株,阳性率22.3%。43株副溶血弧菌共分4个血清群,其中O3,O1,O4居多,分别占60.5%,23.3%和14%。toxR基因检测均阳性,毒力基因分布以tdh阳性和trh阴性为主,占93%(40/43)。结论:沈阳地区副溶血弧菌以O3血清为主,tdh阳性和trh阴性为主要毒力基因型,tdh阴性和trh阳性(或阴性)较少。
Objective: To understand the infection of Vibrio parahaemolyticus in diarrhea patients in Shenyang area. Methods: Diarrhea specimens from local hospitals were collected and tested for virulence genes by isolation and culture, biochemical identification, serotyping and PCR. Results: Among 193 samples of diarrhea, 43 strains of Vibrio parahaemolyticus were detected, the positive rate was 22.3%. 43 isolates of Vibrio parahaemolyticus were divided into 4 serogroups, of which O3, O1 and O4 were the most common, accounting for 60.5%, 23.3% and 14% respectively. toxR gene test were positive, virulence gene distribution to tdh positive and trh negative, accounting for 93% (40/43). Conclusion: Vibrio parahaemolyticus is predominant in O3 in Shenyang, with tdh-positive and trh-negative as the major virulence genotypes, but less tdh-negative and trh-positive (or negative).