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目的探讨宫颈上皮内瘤变患者热圈切除手术(LEEP)后检测人乳头状瘤病毒(HPV)的临床价值。方法第二代杂交捕获试验(Hc2)技术检测LEEP术治疗的198例宫颈上皮内瘤变(CIN)患者宫颈分泌物中的HPV-DNA含量,了解其临床价值。结果术后6个月CINⅠ、CINⅡ、CINⅢ的HPV-DNA检测阳性率均显著低于治疗前(P<0.01),术后18个月时则再次显著降低(P<0.01);CIN病变残留率及复发率在LEEP术前HPV-DNA检测阳性与阴性患者比较差异均无统计学意义(均P>0.05),而术后6个月时HPV-DNA检测阳性患者CIN残留率、复发率均显著高于HPV-DNA检测阴性患者(P<0.01)。结论 CIN患者LEEP术后HPV-DNA检测在判断治疗效果、HPV清除效果及预测病变残留或复发等方面均具有较高价值。
Objective To investigate the clinical value of detecting human papillomavirus (HPV) after thermal coil resection (LEEP) in patients with cervical intraepithelial neoplasia. Methods The second generation hybridization capture assay (Hc2) was used to detect HPV-DNA levels in cervical secretions of 198 cases of cervical intraepithelial neoplasia (CIN) treated with LEEP and its clinical value. Results The positive rates of HPV-DNA in CINⅠ, CINⅡ and CINⅢ at 6 months after operation were significantly lower than those before treatment (P <0.01), and again at 18 months after operation (P <0.01). The residual rate of CIN (P> 0.05). However, the rate of recurrence and the rate of CIN in HPV-DNA positive patients after 6 months of surgery were significantly higher than those of HPV-DNA positive patients Higher than HPV-DNA negative patients (P <0.01). Conclusion The detection of HPV-DNA in patients with CIN after LEEP has high value in judging the therapeutic effect, HPV eradication effect and predicting the residual or recurrence of lesions.