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南方菜豆花叶病毒酶联检测试剂盒 ,采用直接双抗体夹心法。主要由已包被特异性抗体的酶联板、碱性磷酸酯酶标记的抗体、对硝基苯磷酸盐底物以及浓缩清洗液等几部分组成。该试剂盒的检测灵敏度可达 8ng/ml提纯病毒。检测大豆病叶可达 1 :1 0 0 0 ,检测大豆病种子可达 1 :1 0 0 ,与黄瓜花叶病毒 ,马铃薯Y病毒 ,烟草环斑病毒 ,烟草花叶病毒呈阴性反应 ,同时选用大豆等 5种健康植物作阴性对照 ,OD值读数均较低 ,背景反应好 ,未出现假阳性反应。作者对检测样品及酶标抗体的孵育时间进行摸索 ,建议样品孵育时间不宜超过 40h ,酶标抗体的孵育时间以 4h为好 ,本试剂盒的酶联板上包被有特异性抗体 ,可直接使用 ,在真空密封干燥条件下 4℃保存 ,1年内仍然有效。
Southern bean mosaic virus enzyme detection kit, using a direct double antibody sandwich method. Mainly by the specific antibodies have been coated with enzyme-linked board, alkaline phosphatase-labeled antibodies, nitrophenyl phosphate substrate and concentrated cleaning solution and other parts. The kit has a detection sensitivity up to 8ng / ml purified virus. Detection of soybean leaves up to 1: 1 0 0 0, the detection of soybean seed up to 1: 1 0 0, and cucumber mosaic virus, potato virus Y, tobacco ring spot virus, tobacco mosaic virus was negative reaction, while selecting Soybean and other five kinds of healthy plants as a negative control, OD value readings are low, the background reaction is good, no false positive reaction. The authors explored the incubation time of the test sample and enzyme-labeled antibody, suggesting that the incubation time of the sample should not exceed 40 hours and the incubation time of the enzyme-labeled antibody should be 4 hours. The kit is coated with specific antibody on the enzyme-linked plate and can be directly Use, stored in a vacuum sealed dry at 4 ℃, still valid within 1 year.