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本研究应用四血管结扎大鼠全脑缺血模型，借助放射免疫分析法，观察了大鼠脑缺血后皮层亮－脑啡肽（ＬＥＫ）、β－内啡肽（β－ＥＰ）和精氨酸加压素（ＡＶＰ）含量的动态变化，在此基础上进一步观察了预先侧脑室注射亮－脑啡肽抗血清（ＬＥＫ－ＡＳ）对脑缺血后皮层β－ＥＰ、ＡＶＰ含量的影响。结果显示：ＬＥＫ的含量在脑缺血早期明显升高而后迅速降低，β－ＥＰ、ＡＶＰ则随着缺血时间的延长逐渐升高；预先予侧脑室注射ＬＥＫ－ＡＳ后，缺血皮层β－ＥＰ、ＡＶＰ的含量明显降低。研究结果提示：ＬＥＫ、β－ＥＰ、ＡＶＰ均参与脑缺血的病理生理过程，ＬＥＫ可能通过影响ＡＶＰ或／和β－ＥＰ而间接参与脑缺血的病理生理过程。
In this study, the model of global cerebral ischemia was established by ligating the blood vessels of four rats. Radioimmunoassay was used to observe the changes of leukoencephalitis (LEK), β-endorphin (β-EP) (AVP), we further observed the effect of pre-administration of LEK-AS on the content ofβ-EP and AVP in cerebral cortex after cerebral ischemia . The results showed that: LEK content increased significantly in the early stage of cerebral ischemia and then decreased rapidly, β-EP, AVP with the increase of ischemic time gradually increased; pre-intraventricular administration of LEK-AS after ischemic cortex β- EP, AVP content was significantly reduced. The results suggest that LEK, β-EP and AVP all participate in the pathophysiological process of cerebral ischemia. LEK may indirectly participate in the pathophysiological process of cerebral ischemia by affecting AVP or / and β-EP.