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目的:研究小檗碱在金黄色葡萄球菌(S.aureus)诱导ECV-304细胞内凋亡相关基因Bcl-2、Bax和Caspase-3表达的调节作用,探讨其抑制金黄色葡萄球菌诱导ECV-304细胞凋亡的机制。方法:小檗碱(Berberine,BBR)预处理ECV-304细胞2h后,用1∶100感染复数的S.aureus感染细胞,采用半定量RT-PCR及Western-blot法检测金黄色葡萄球菌感染后ECV-304细胞内Bcl-2、Bax和Caspase-3的mRNA及蛋白表达水平。结果:小檗碱预处理ECV-304后再感染S.aureus可显著性增加凋亡相关细胞因子Bcl-2 mRNA水平及蛋白表达量,而Bax和Caspase-3表达下调。结论:小檗碱可以通过调节凋亡相关基因Bcl-2、Bax和Caspase-3的表达抑制S.aureus诱导的ECV-304细胞凋亡,本实验结果为临床治疗S.aureus感染提供理论依据。
AIM: To investigate the regulatory effect of berberine on the expression of apoptosis-related genes Bcl-2, Bax and Caspase-3 in S. aureus induced ECV-304 cells and to investigate the inhibitory effect of berberine on the expression of ECV- 304 cell apoptosis mechanism. Methods: ECV-304 cells were pretreated with berberine (BBR) for 2 hours and then infected with S.aureus at a multiplicity of infection of 1: 100. Semi-quantitative RT-PCR and Western-blot were used to detect the infection of S. aureus The mRNA and protein expression of Bcl-2, Bax and Caspase-3 in ECV-304 cells. RESULTS: Berberine pretreatment with S. aureus followed by ECV-304 significantly increased the mRNA and protein expression of Bcl-2, while the expressions of Bax and Caspase-3 were down-regulated. Conclusion: Berberine can inhibit the apoptosis of ECa-304 cells induced by S. aureus through regulating the expression of apoptosis related genes Bcl-2, Bax and Caspase-3. The results provide a theoretical basis for clinical treatment of S. aureus infection.