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目的:建立同时测定莲菊感冒胶囊中1,5-O-二咖啡酰基-奎宁酸、3,5-O-二咖啡酰基-奎宁酸、3,4-O-二咖啡酰基-奎宁酸的超高效液相色谱(UPLC)分析方法。方法:采用Waters Acquity UPLC系统,Ac-quity UPLC BEHC18色谱柱(100 mm×2.1mm,1.7μm),流动相为乙腈-0.1%磷酸,以流速为0.2 mL.min-1进行梯度洗脱;柱温:45℃;样品经50%乙醇超声提取后用UPLC-PDA进行分析检测,检测波长为325 nm。结果:1,5-O-二咖啡酰基奎宁酸、3,5-O-二咖啡酰基奎宁酸、3,4-O-二咖啡酰基奎宁酸3种被测成分在线性范围内均具有良好的线性关系(r≥0.999 8);平均回收率在99.1%~101.7%之间,RSD≤2.5%。结论:UPLC分离效果及重复性好,且快速、简便,可作为莲菊感冒胶囊的质量控制方法。
OBJECTIVE: To establish a method for the simultaneous determination of 1,5-O-disaccharidyl-quinic acid, 3,5-O-disuccinacyl-quinic acid, 3,4-O-disofoacyl- Analysis of acid by high performance liquid chromatography (UPLC). Methods: The Waters Acquity UPLC system was used. Ac-quity UPLC BEHC18 column (100 mm × 2.1 mm, 1.7 μm) was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid and eluted with a gradient of 0.2 mL · min- Temperature: 45 ℃. The samples were extracted by 50% ethanol and analyzed by UPLC-PDA. The detection wavelength was 325 nm. Results: The three tested components of 1,5-O-decalciferylquinic acid, 3,5-O-disaccharidylquinic acid and 3,4-O-disaccharidylquinic acid all showed linearity (R≥0.999 8). The average recoveries ranged from 99.1% to 101.7% with RSD ≤ 2.5%. Conclusion: UPLC separation and reproducibility is good, fast and simple, and can be used as a quality control method for lotus capsules.